Dental Pulp Stem Cells (DPSCs) have been identified as a rich source of stem cells, obtained from teeth and therefore easily accessible in the oral cavity (1). These cells are unspecialized, can replicate, and also have the potential to differentiate into more specific cell types, tissue or organs (2). DPSCs exhibit ‘Mesenchymal stem cell’ (MSC) like qualities found in bone marrow, capable to be induced in vitro to differentiate into osteoblasts (bone cell), astrocytes (cell of brain and spinal cord) (3). These cells have high growth potential and proliferation rates than stem cells from other tissue sources. (4,5) Hence, they can be potentially used to treat a range of disorders and conditions such as bone pathologies, osteoarthritis, spinal cord injuries and many more. In addition, unlike other stem cells, DPSCs exhibit low immunogenicity and suppress alloreactive T cell response (transplanted allogenic MSC are not rejected) (6). DPSCs have been shown to express developmentally important transcription factors, such as oct4, sox2, klf4, nanog and c-myc, which is an indicator of their pluripotency.
DPSCs can be collected from teeth of patients from various age groups. Deciduous teeth or baby teeth are regarded to be the best source of dental stem cells. Nevertheless they can be recovered from patients who are middle aged, but the younger they are the better. The tooth needs to have an intact blood supply and be free from infection and deep cavities. These cells are then stored in a cryopreservation facility until it required by the patient.
Dental pulp stem cells have drawn attention in recent years because of their accessibility, plasticity and high proliferative ability.
The dental pulp derived mesenchymal stem cells (DPSCs) are isolated from healthy human premolars / third molars collected during an orthodontic procedure or during the extraction of a ‘wisdom’ tooth. DPSCs are cryopreserved at primary passage with not less than 1 million cells per vial. These cells have demonstrated very similar phenotypic and functional characteristics to that of bone marrow-derived mesenchymal stem cells. DPSCs have been reported to differentiate into many different lineages including chondrogenic, osteogenic, adipogenic and neural.
Unpacking and Storage Instructions
Thawing of cells / Initiation of the culture process.
|Product subcategory||Primary cells|
|Intended use||Research and development|
|Passage Level||Passage 0-5|
|No. of Viable Dental Pulp cells||1 million cells/vial|
|Cell Morphology||Fibroblast like spindle shaped cells|
|MSC cell count||≥1 million frozen cells per cryovial|
Warning: THESE PRODUCTS ARE FOR RESEARCH USE ONLY. Not approved for human or veterinary use, for application in humans or animals, or for use in clinical or in vitro procedures.