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Clinical Outcomes of xeno-free autologous cultivated limbal epithelial transplantation :a 10-year study

Virender S Sangwan , Sayan Basu, Geeta K Vemuganti , Kunjal Sejpal , Sandhya V subramaniam , Souvik Bandyopadhyay , Sannapaneni Krishnaiah , Subhash Gaddipai, Shubha Tiwari, Dorairajan Balasubramanian

September 2, 2011 as 10.1136/bjophthalmol-2011-300352

LV Prasad Eye

Institute Efficacy of xeno-free autologuous cell- based treatment of limbal stem cell deficiency. A completely epithilised , avascular and clinically stable coreneal surface was seen in 142 of 200(71%) eyes at a mean follow-up of 3 + or – 1.6(range :1-7.6)years. Over the last two decades the surgical techniques has evolved from direct limbal transplantation to transplantation of ex vivo cultivated limbal epithelical cells, limbal cultivation may involve use of xenogenic tissue , associated with the risk of transmission of known or unknown infections to the transplant recipient of murine feeder cells, fetal bovine serum(FBS) or animal-derived growth factors. We report the long term survival, visual benefit and complications of transplantation of autologous limbal epithelial cells.


Stem cells in clinical practice: applications and warnings

Lodi et al. Journal of Experimental & Clinical Cancer Research 2011, 30:9

Daniele Lodi1 , Tommaso lannitti2*, Beniamno Palmieri3

A cell polarizes itself, so that cell-fate determinant molecules are specifically localized on one side. After that, the mitotic spindle aligns itself perpendicular to the axis polarity. End of the process two different cells are obtained [5-7]. Promising results have been described in disorders, such as diabetes [273] and neurodegenerative diseases [274, 275], where SCs graft can reestablish one or more deficit cellular lineages and, generally, a healthy state. Notably, many clinical studies have underlined the immunomodulatory effect of SCs in autoimmune diseases, such as multiple sclerosis [275], organ transplants [276] and in uncontrolled immune-inflammatory reactions [277-279].


Cardiovascular Applications of Stem Cell Therapy

Soheil Sadri1 , Ramesh Mazhari2, Maziar Sadri2, Nahal Konjedi1, and Palak Shah2*

Sadri et al.. J Stem Cell Res Ther 2011. S1

The prospect of cell-based therapies for cardiovascular disease has led to excitement within the scientific community as cardiovascular disease continues to be the leading cause of death worldwide. There is a growing body of evidence from both the basic sciences as well as through translational studies that prove the ability of stem cells to improve left ventricular function.


Mesenchymal stem cell-based therapies in regenerative medicine: application in rheumatology

Marie Maumus 1.2, David Guerit 1.2 , Karine Toupet 1.2, Christan Jorgensen 1.2.3 and Daniele Noel*1.2.4

Maumus et al. Stem Cell Research & Therapy 2011, 2:14

Growing knowledge on the biology of mesenchymal stem cells (MSCs) has provided new insights into their potential (currently under experimental research) clinical applications, particularly for rheumatologic disorders. Historically, their potential to differentiate into cells of the bone and cartilage lineages has led to a variety of experimental strategies to investigate whether MSCs also display immunosuppressive properties, which have prompted research on their capacity to suppress local inflammation and tissue damage in a variety of inflammatory autoimmune diseases and, in particular, in rheumatoid arthritis. Currently, an emerging field of research comes from the possibility that these cells, through their trophic/regenerative potential (currently under experimental research), may also influence the course of chronic degenerative disorders and prevent cartilage degradation in osteoarthritis.


Human Dental Pulp Stem Cells Hook into Biocoral Scaffold Forming an Engineered Biocomplex

Carlo Mango1', Francesca Paino2', Riccardo d' Aquino2 , Alfredo De Rosa3 , Giovanna lezzi4 , Adriano

Piattello4, Luigi Laino3, Thimios Mitsiadis5 , Vincenzo Desiderio2 , Francesco Mangano1, Gianpalolo

Papaccio2*, Virginia Tirino2

The aim of this study was to evaluate the behavior of human Dental Pulp Stem Cells (DPSCs), as well as human osteoblasts, when challenged on a Biocoral scaffold, which is a porous natural hydroxyapatite. For this purpose, human DPSCs were seeded onto a three-dimensional (3D) Biocoral Scaffold or on flask surface (control). Either normal or rotative (3D) cultures were performed. Scanning electron microscopic analyses, at 8, 24 and 48 h of culture showed that cells did not adhere on the external surface, but moved into the cavities inside the Biocoral structure. After 7, 15 and 30 days of culture, morphological and molecular analyses suggested that the Biocoral Structure. After 7, 15 and 30 days of culture, morphological and molecular analyses suggested that the Biocoral scaffold leads DPSCs to hook into the cavities where there cells quickly start to secrete the extra cellular matrix (ECM) and differentiate into osteoblasts. Controls human osteoblasts also moved into the internal cavities where they secreted the ECM. Histological sections revealed a diffuse bone formation inside the Biocoral samples seeded with DPSCs or human osteoblasts, where the original scaffold and the new secreted biomaterial were completely integrated and cells were found within the remaining cavities. In addition, RT-PCR analyses showed a significant increase of osteoblasts-related gene expression and above all, of those genes highly expressed in mineralized tissues, including osteocalcin, OPN and BSP. Furthermore, the effects on the interaction between osteogenesis and angiogenesis were observed and substantiated by ELISA assays. Taken together, our results provide clear evidence that DPSCs differentiated into osteoblasts, forming a biocomplex made of Biocoral, ECM and differentiated cells.


Dental Pulp Stem Cells and Tissue Engineering Strategies for Clinical Application on Odontoiatric Field

Zavan Barbara et al. University of Padova Italy

The dental pulp plays a major role in tooth regeneration after injury, participating process called reparative dentinogenesis.


Human dental pulp stem cell is a promising autologous seed cell for bone tissue engineering

LI Jing-hui, LIU Da-yong, ZHANG Fang-ming, WANG Fan, ZHANG Wen-kui and ZHANG Zhen-ting

Chin Med J 2011;124(23):4022-4028

In vitro studies revealed that hDPSCs do possess osteogenic differentiation potential .In vivo studies revealed that hDPSCs seeded on gelatin scaffolds can form bone structure in heterotopic sites of nude mice.

These findings suggested that hDPSCs may be valuable as seed cells for bone tissue engineering .As a special stem cell source ,hDPSCs may be blaze a new path for bone tissue engineering.


Odontogenic Differentiation of Human Dental pulp stem cells stimulated by the Calcium Phosphate Porous Granules

Sunyoung Nam, Jong-Eun Won, Cheol-Hwan Kim, and Hae –Won Kim

Effects of three-dimensional (3D) calcium phosphate (Cap) porous granules on the growth and odontogenic diffentiation of human dental pulp stem cells (hDPSCs) were examined for dental tissue engineering .hDPSCs isolated from human dental pulps were cultured for 3-4 passages, and populated on porous granules .Cell growth on the culture dish showed on ongoing increase for up to 21 days, whereas the growth on the 3D granules decreased after 14 days. This reduction in proliferative potential on the 3D granules was more conspicuous under the osteogenic medium conditions, indicating that the 3D granules may alkaline phosphatase activity, up-regulation of odontoblast- specific genes, including dentin sialosphosprotein (DSPP) and dentin matrix protein 1(DMPI) by quantitative polymerase chain reaction , and greater level of dentin sialoprotein synthesis by western blot. Moreover, the cellular mineralization, as assessed by Alizarin red S and calcium quantification, was significantly higher in the 3D CaP granules than in the culture dish. Taken all, the 3D CaP porous granules should be useful for dental tissue engineering in combination with hDPSCs by providing favorable 3D substrate conditions for cell growth and odontogenic development.


Stem cell-based treatments for Type 1 diabetes mellitus:

bone marrow, embryonic, hepatic, pancreatic and induced pluripotent stem cells

K. J. Godfrey1, B. Mathew1, J. C. Bulman1 , O. Shah1 , S. Clement2 and G. l. Gallicano3

Type I diabetes mellitus-Characterized by the permanent destruction of insulin-secreting β-cells-is responsive to cell-based treatment that replace lost β-cell populations. The current gold standard of pancreas transplantation provides only temporary independence from exogenous insulin and is fraught with complications, including increased mortality. Stem cells offer a number of theoretical advantages over current therapies. Our review will focus on the development of treatments involving tissue stem cells from bone marrow, liver and pancreatic cells, as well as the potential (currently under experimental research) use of embryonic and included pluripotent stem cells for Type 1 diabetes therapy. These cells have been demonstrated to increase endogenous insulin production, while partially mitigating the autoimmune destruction newly formed β-cells.

Diabet. Med. 29, 14-23 (2012)


Sinus Lift Augmentation Using Autologous Pulp Stem Cells: Case Report of Bone Density Evaluation

CAP/D/2011/Sep 1



Don Orione Hospital, Bergamo, Italy
(Applied Medical Imaging Research Group), Milan, Italy
Department of D.M.C.C.,Section of Maxillofacial and Plastic Surgery, University of Ferrara, Ferrara, Italy

Bone augmentation to reconstruct atrophic jaws provides the base for sufficient functional and aesthetic implant-supported oral rehabilitation. Although autografts are the standard procedure for bone grafting, the use of bone regeneration by means of dental pulp stem cell is an alternative that open a new era in this field. In March 2010, at the Department of Oral Surgery, Don Orione Hospital, Bergamo, Italy, one patient undergo to sinus lift elevation with pulp stem cells gentle poured onto collagen sponge. A CT scan control was performed after 4 month and DICOM data were processed with medical imaging software which gives the possibility to use a virtual probe to extract the bone density. Pearson's chi-square test was used to investigate difference in bone density (i.e. BD) between native and newly formed bone. BD in newly formed bone is about the double of native bone. This report demonstrated that stem cells derived from dental pulp poured onto collagen sponge is a useful method for bone regeneration in atrophic maxilla.


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