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2008

Human CD34+ Stem Cells Produce Bone Nodules in Vivo

PRC/D/2008/Feb/1

Cell Prolif.2008, 41

A. Graziano, R. d'Aquino, G. Laino, A. Proto, M. T. Giuliano, G. Pirozzi, A. De Rosa, D. Di Napoli and G. Papaccio

Dipartimento di Medicina Sperimentale, Sezione Istologia ed Embriologia, TESLab, Secondo Ateneo di Napoli, Napoli, Italy, Dipartimento di Discipline Odontostomatologiche, Ortodontiche e Chirurgiche, Secondo Ateneo di Napoli, Napoli, Italy, Dipartimento di Chimica Università degli Studi di Salerno, Salerno, Italy, Dipartimento di Medicina Sperimentale, Sezione di Biotecnologie, Secondo Ateneo di Napoli, Napoli, Italy, Oncologia Sperimentale C-Immunologia, Istituto Nazionale Tumori, Napoli, Italy, and Centro di Biotecnologie, AORN Cardarelli, Napoli, Italy

Objectives: The aim of this study was to select and provide enough stem cells for quick transplantation in bone engineering procedures.

Materials and Methods: germ pulp, collected from 25 healthy subjects aged 13–20 years, were subjected to magnetic-activated cell sorting to select a CD34+ Stem cell population capable of differentiating into pre-osteoblasts. These cells were allowed to adhere to an absorbable polylactic–coglycolic acid scaffold for 30 min, without any prior expansion, and the CD34+ cell-colonized scaffolds were then transplanted into immunocompromised rats, subcutaneously.

Results: After 60 days, analysis of recovered transplants revealed that they were formed of nodules of bone, of the same dimensions as the original scaffold.

This study indicates that CD34+cells obtained from dental pulp can be used for engineering bone, without the need for prior culture expanding procedures. Using autologous stem cells, this schedule could be used to provide the basis for bone regenerative surgery, with limited sacrifice of tissue, low morbidity at the collection site, and significant reduction in time needed for clinical recovery.

 

Human Dental Pulp Stem Cells Improve Left Ventricular Function, Induce Angiogenesis, and Reduce Infarct Size in Rats with Acute Myocardial Infarction

PRC/D/2008/Mar 2

STEM CELLS

CAROLINA GANDIA

Unidad de Cardiorregeneracio´n, Centro de Investigacio´n Prı´ncipe Felipe, Valencia, Spain

ABSTRACT

Human dental pulp contains precursor cells termed dental pulp stem cells (DPSC) that show self-renewal and multilineage differentiation and also secrete multiple proangiogenic and antiapoptotic factors. To examine whether these cells could have therapeutic potential (currently under experimental research) in the repair of myocardial infarction (MI), DPSC were infected with a retrovirus encoding the green fluorescent protein (GFP) and expanded ex vivo. Seven days after induction of myocardial infarction by coronary artery ligation, 1.5 _ 106 GFP-DPSC were injected intramyocardially in nude rats. At 4 weeks, cell-treated animals showed an improvement in cardiac function, observed by percentage changes in anterior wall thickening left ventricular fractional area change, in parallel with a reduction in infarct size. No histologic evidence was seen of GFP+ endothelial cells, smooth muscle cells, or cardiac muscle cells within the infarct. However, angiogenesis was increased relative to control-treated animals. Taken together, these data suggest that DPSC could provide a novel alternative cell population for cardiac repair, at least in the setting of acute MI. STEM CELLS 2008;26:638–645

 

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